中
Biography

Dr Gang CHEN is an Associate Professor in the School of Medicine, The Chinese University of Hong Kong, Shenzhen (CUHK-Shenzhen). He received his B.S. degree in Chemistry at the University of Science and Technology of China (USTC) in 2001. He did his Ph.D. studies with Prof. Douglas TURNER in the Department of Chemistry at the University of Rochester. His Ph.D. work involved thermodynamic and NMR studies of RNA internal loops. A better understanding of the sequence dependence of thermodynamics for RNA structures will improve the accuracy of the RNA secondary structure prediction programs such as MFOLD and RNAstructure. He earned his Ph.D. in 2005. He was a postdoctoral fellow in Prof. Ignacio TINOCO’s lab in the Department of Chemistry at the University of California, Berkeley from January 2006 to June 2009. His research in TINOCO lab was on single-molecule mechanical unfolding and folding of RNA pseudoknots by laser optical tweezers, which provided new insights into ribosomal reading-frame regulation by cis-acting mRNA structures. He was a Research Associate in Prof. David MILLAR's lab in the Department of Molecular Biology at The Scripps Research Institute working on HIV-1 Rev-RRE assembly using single-molecule fluorescence techniques. In July 2010, he joined the faculty in the Division of Chemistry and Biological Chemistry at Nanyang Technological University in Singapore. He joined MED at CUHK-Shenzhen in 2020. 

His team has been focused on (1) using biophysical and biochemical methods (including single-molecule manipulation using high-resolution optical tweezers) for probing the  molecular interactions accounting for the structures, stabilities, dynamics, and functions of RNAs and RNA-ligand complexes; and (2) developing RNA structure-targeting programmable chemically modified peptide nucleic acids (PNAs) and other functional molecules as chemical probes, diagnostic tools, and therapeutic drugs. The team’s high-impact research on targeting RNA duplexes has generated significant interests in the RNA community. The team has been invited to contribute to (1) a review article on RNA triplexes in the journal Wiley Interdisciplinary Reviews: RNA, (2) a book chapter (published by Springer) on recognition and targeting of mature miRNA and miRNA hairpin precursor by duplex and triplex formation, respectively, (3) a methods article for the Journal of Visualized Experiments on sequence-specific and selective recognition of double-stranded RNAs over single-stranded RNAs by chemically modified peptide nucleic acids, (4) a News & Views article for the journal Nature Chemical Biology on the importance of base triples, junctions, and other non-Watson-Crick interactions in facilitating catalytic reactions of an RNA enzyme, and (5) an article in the Future of Biochemistry special issue in the journal Biochemistry (invited by the Editor-In-Chief, Prof Alanna Schepartz). The interdisciplinary team welcomes talents to join us to probe and target RNA sequences and structures with the ultimate goals of developing modern biological tools, disease diagnosis methods, and precision medicines. 


Research

Research

The overall goal of Dr. CHEN’s research group in the School of Medicine, at The Chinese University of Hong Kong, Shenzhen (CUHK-Shenzhen) is to better understand the structures and the physical-chemical properties of RNAs and RNA-ligand complexes to provide deeper insight into and to facilitate precise control of the diverse biological functions involving RNA. We aim to use the fundamental knowledge to fight neurodegenerative diseases, cancers, bacterial and viral infections by designing and discovering novel therapeutic ligands targeting RNA. To approach the challenging goals, we have assembled a multidisciplinary team with expertise ranging from molecular biophysics, structural biology, computation, chemical synthesis, cell biology, animal model studies, to medical healthcare. The research projects of current interests are: (1) characterizing the molecular recognition interactions (e.g., hydrogen bonding and aromatic base stacking) accounting for structure, stability, and dynamics of RNA structural building blocks such as internal loops, hairpins, triplexes, and pseudoknots, (2) probing the complex energy landscapes of RNA folding and assembly with protein, (3) designing and discovering therapeutic ligands (small molecules, oligonucleotides, peptides, peptide nucleic acids, etc.) targeting RNA, (4) developing nucleic acid based nano-biosensors to rapidly detect toxic/pathogenic agents in food products and human body, and (5) discovering and characterizing novel nucleic acid based nano-catalysts for important organic and inorganic reactions at mild conditions.

 

We employ various conventional and cutting-edge techniques including laser optical tweezers, NMR, UV-Vis absorption, fluorescence, SPR, gel electrophoresis, PCR, chemical synthesis of modified oligonucleotides and peptides, in vitro transcription, protein expression, and cell culture assay. The research experience in the laboratory will help the students to grasp fundamental knowledge and experimental skills, to develop learning skills such as rigorous reasoning and innovative thinking, and to be able to ask and answer important questions within and beyond chemical and molecular sciences.

 

Research Grants:

Research Grants at CUHK-Shenzhen:

PI, 2022-2025, The National Natural Science Foundation of China, General Program

University Development Fund

External Grants at NTU

PI, Nov 2019 – Oct 2022 (terminated in Sep 2020), Detecting and targeting influenza viral panhandle RNA structure, S$604,730 (with an additional Indirect Cost of S$120,946), MOE AcRF Tier 2

Co-PI, Jan 2019 – Jan 2022, Role of RNA editing in guarding against hepatic injury and hepatocarcinogenesis, S$15,000 (S$687,500), MOE AcRF Tier 2

PI, Jan 2016 – Dec 2018, Targeting pre-mRNA and pre-miRNA by RNA duplex-binding peptide nucleic acids, S$554,327 (with an additional Indirect Cost of S$110,865), MOE AcRF Tier 2

PI, Apr 2014 – Mar 2017, Unravelling mRNA structure’s role in translational regulation, S$542,534 (with an additional Indirect Cost of S$108,507), MOE AcRF Tier 2

 

Internal Grants at NTU

PI, Apr 2018 – Mar 2019, Developing near-infrared fluorescent light-up probes for detecting RNA structures in viruses and cancers, S$40,000, NTU-A*STAR Seed Funding Research Award

PI, Mar 2018 – Feb 2020, Tagging CRISPR guide RNAs, S$99,999, MOE AcRF Tier 1

PI, Nov 2015 – Nov 2017, Creating high-information-content nano-structures based on modified nucleic acids, S$99,999, MOE AcRF Tier 1

 

PI, Nov 2013 – Oct 2015, Synthesis of modified triplex-forming peptide nucleic acids for targeting HIV-1 ribosomal frameshift stimulatory RNA structure, S$99,999, MOE AcRF Tier 1

PI, Mar 2011 – Feb 2014, Targeting RNA duplexes with modified triplex-forming oligonucleotides: From atomic mutation to single-molecule manipulation, S$200,000, MOE AcRF Tier 1

Co-PI, Jan 2011 – Dec 2013, Solid state nanopore devices for single molecule biophysics and sequencing, S$25,000 (S$75,000), SPMS Collaborative Research Award

PI, Jul 2010 – Dec 2022, RNA structures, dynamics, and function,  S$269,700, Nanyang Technological University Start-Up Grant

Short summary of our research:

  • RNAs become increasingly important disease biomarkers and drug targets. RNAs often form complex secondary structures containing both single-stranded (ss) loop and double-stranded (ds) stem regions, with the dsRNAs mainly stabilized by Watson–Crick base pairing and stacking interactions. By nano-manipulation using optical tweezers in combination with other biophysical methods, we have revealed at the single-molecule level the contributions of molecular recognition interactions such as a single hydrogen bond in a base pair or base triple to RNA folding and unfolding and characterized the effects of a single proton binding. We have unveiled the correlations (i) between mRNA mechanical properties and mRNA structure induced translational reading frame shifting and (ii) between mechanical properties of tau pre-mRNA splice site hairpin structures and pre-mRNA alternative splicing activities.

  • In addition, we are developing a molecular recognition platform based on chemically modified Peptide Nucleic Acids (PNAs) for the targeting of RNA structures in a sequence- and structure-specific manner. Specifically, we program our dsRNA-binding PNAs (dbPNAs) with a new four-letter chemical code (T, L or R, E or S, and Q) for the recognition of RNA Watson–Crick duplexes, through the formation of major-groove triples. We have demonstrated that short (e.g., 10-mer) PNAs containing L, R, E, S, or Q show enhanced sequence-specific recognition of RNA base pairs in dsRNAs with significantly weakened binding to ssRNAs or dsDNAs at near-physiological conditions. Our cell culture studies show that our PNAs conjugated with small molecules are bioactive in the targeting of viral and cellular RNAs. Thus, we will further develop our dbPNAs platform as useful diagnostic tools and therapeutic drugs to fight against viral infections, cancers, and neurodegenerative diseases. 

 

Our research contribution and impact:

Chemically-modified short (e.g., 10-mer) PNA oligomers that sequence-specifically bind to dsRNAs are promising complementary tools of traditional antisense strands and small molecules for viral inhibition and regulation of cellular functions. The dsRNA regions are often relatively rigid and may not be easily accessible by traditional antisense oligonucleotides and small molecules, which are usually ideal for targeting non-Watson-Crick regions such as junctions, bulges, and internal loops. For example, formation of PNA·dsRNA triplexes has been shown to result in the selective inhibition of translation (work by Eriks ROZNERS lab from Binghamton University, USA) and ribosomal frameshifting (work by our lab), respectively, through steric blockage and/or stabilization of RNA duplex structures. The relatively short length (10-mer) of our dsRNA-binding PNA may also minimize the solubility problem observed for some longer PNAs.

 

The dsRNA-binding PNAs platform is programmable and may allow us to design PNAs binding to many functionally important viral and cellular RNA structures. Our research will have a significant impact on the field of the RNA interactome, specifically pertaining to the identification and targeting of dsRNA structures important in RNA biology and pathology. Further demonstration of the applications our PNAs platform in the targeting of various biomedically important RNA structures in cell cultures and animal models will help establish industrial collaborations to translate our laboratory ideas into real products.

 

Our high-impact research on targeting RNA duplexes has generated significant interests in the RNA community. We wrote an invited review article on RNA triplexes in the journal Wiley Interdisciplinary Reviews: RNA. We also wrote an invited book chapter (published by Springer) on recognition and targeting of mature miRNA and miRNA hairpin precursor by duplex and triplex formation, respectively. We were also invited to write a methods article for the Journal of Visualized Experiments on sequence-specific and selective recognition of double-stranded RNAs over single-stranded RNAs by chemically modified peptide nucleic acids. We wrote a News & Views article for the journal Nature Chemical Biology on the importance of base triples, junctions, and other non-Watson-Crick interactions in facilitating catalytic reactions of an RNA enzyme. We were invited by the editor-in-chief of Biochemistry (Alanna Schepartz from Yale University, USA) to contribute a paper to the Future of Biochemistry special issue in Jan 2018. I have been also invited to give keynote and invited talks at conferences.

 

More ++
Awards and honors
  • 2018
  • 2010
  • 2009
  • 2004
  • 2003
<
  • 01

    2018

    Featured as one of 44 scientists worldwide on the cover of a special issue Future of Biochemistry (Jan 2018) in the journal Biochemistry

  • 02

    2010

    Postdoctoral Research Fellowship from California HIV/AIDS Research Program (CHRP) (declined)

  • 03

    2009

    Nature Structural & Molecular Biology Sponsored "Best Poster in Biophysics and Structural Biology" at 2009 RNA Society Meeting

  • 04

    2004

    Recipient, Robert and Marian Flaherty Deright Fellowship, University of Rochester, USA

  • 05

    2003

    Recipient, Sherman-Clark Fellowship, University of Rochester, USA

>
Publications

Online profiles: 

My WeChat ID is RNA-CHEN

ResearcherID  (B-7027-2011)       

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Patents Filed

5.  PCT专利，PCT/CN2024/115426,  Title: 靶向RNA 的组合物及其用途 (Chimeras for targeting RNA and uses thereof) 发明人：陈刚、鲁荣光、邓莉萍、廉云、占轩、杨利霞、林芮羽、范诗健。申请人：香港中文大学（深圳）；香港中文大学（深圳）福田生物医药创新研发中心



4. 中国专利，申请号：202311069752.9, 基于G-四链体的核酸分子及其应用。发明人：陈刚、占轩、廉云、邓莉萍、鲁荣光、王安妮、白诗瑶、蒋成。申请人：香港中文大学（深圳）；香港中文大学（深圳）福田生物医药创新研发中心


3. 中国专利申请号：202311137198.3, 靶向RNA 的组合物及其用途。发明人：陈刚、鲁荣光、邓莉萍、廉云、占轩、杨利霞、林芮羽、范诗健。申请人：香港中文大学（深圳）；香港中文大学（深圳）福田生物医药创新研发中心

2. PCT专利，PCT/SG2020/050380, Title: Inhibitors of RNA Editing and Uses Thereof.  WO2021002805  
National Phase Entry:
Singapore (Dec 23, 2021); 
European Patent Office (Feb 4, 2022);
China Patent Office (Mar 3, 2022);
US Patent Office (December 24, 2022)
Inventors: 1) Leilei CHEN; 2) Daryl Jin Tai TAY; 3) Gang CHEN 
Applicants: 1) National University of Singapore; 2) Nanyang Technological University

1. PCT专利，PCT/SG2015/050319,  Title: Modified Peptide Nucleic Acids And Their Use. 
Inventors: 1) Gitali DEVI; 2) Desiree-Faye TOH Kaixin; 3) Kiran M. PATIL; 4) QU Qiuyu; 5) Manikantha MARASWAMI; 6) Elzbieta KIERZEK; 7) XIAO Yunyun; 8) LOH Teck Peng; 9) ZHAO Yanli; 10) CHEN Gang
Applicants: 1) Nanyang Technological University; 2) Institute of Bioorganic Chemistry Polish Academy of Sciences.


Book Chapters

 1. Patil, K.M., and Chen, G. (2016) Recognition of RNA sequence and structure by duplex and triplex formation: targeting miRNA and pre-miRNA, in Modified Nucleic Acids in Biology and Medicine, (Volker A. Erdmann, Stefan Jurga and Jan Barciszewski, Eds.), Springer series: RNA Technologies. Doi: 10.1007/978-3-319-34175-0_13


 

Journal Papers 

50.     Rongguang Lu, Liping Deng, Yun Lian, Xin Ke, Lixia Yang, Kun Xi, Alan Ann Lerk Ong, Yanyu Chen, Hanting Zhou, Zhenyu Meng, Ruiyu Lin, Shijian Fan, Yining Liu, Desiree-Faye Kaixin Toh, Xuan Zhan, Manchugondanahalli S. Krishna, Kiran M. Patil, Yunpeng Lu, Zheng Liu, Lizhe Zhu, Hongwei Wang, Guobao Li* and Gang Chen* (2024) Recognition of RNA secondary structures with a programmable peptide nucleic acid-based platform. Cell Rep Phys Sci, 5, 102150. (Cell Press, IF: 7.9)

49.     Xuan Zhan, Liping Deng, Yun Lian, Zhiyu Shu, Yunong Xu, Xinyi Mai, Manchugondanahalli S. Krishna, Rongguang Lu, Anni Wang, Shiyao Bai, Fangyu Zhou, Chi Xiong, Yingyi Xu, Jie Ni, J. Jeya Vandana, Dongmei Sun, Shaohui Huang, Jingyan Liu, Gui-Juan Cheng, Song Wu, Ying-Chih Chiang,* Stjepanovic Goran,* Cheng Jiang,* Yong Shao* and Gang Chen* (2024) Enhanced Recognition of a Herbal Compound Epiberberine by a DNA Quadruplex-Duplex Structure. Anal Chem, 96, 13174-13184.(ACS journal, IF: 7.4) 


48.     Rongguang Lu, Gang Chen* (2024) Pre-twisting for improved genome modification and miRNA targeting. Trends Biochem Sci, 49, 283-285. Spotlight article (Cell Press, IF: 14.264) 


47.     Xuan Zhan, Liping Deng, and Gang Chen* (2022) Mechanisms and applications of peptide nucleic acids (PNAs) selectively binding to dsRNA. Biopolymers, 113, e23476.

Invited contribution to a special issue on 30 years of PNA.


46.     Daryl Jin Tai Tay, Yangyang Song, Boya Peng, Tan Boon Toh, Lissa Hooi, Desiree-Faye Kaixin Toh, Huiqi Hong, Sze Jing Tang, Jian Han, Wei Liang Gan, Tim Hon Man Chan, Manchugondanahalli S. Krishna, Kiran M. Patil, Manikantha Maraswami, Teck Peng Loh, Yock Young Dan, Lei Zhou, Glenn Kunnath Bonney, Pierce Kah-Hoe Chow, Gang Chen, Edward Kai-Hua Chow, Minh TN. Le, Leilei Chen* (2021) Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer. Mol Ther, 29, 3258-327. (IF: 8.986)


45.     Arijit Maity, Fernaldo Richtia Winnerdy, Gang Chen, and Anh Tuân Phan* (2021) Duplexes formed by G4C2 repeats contain alternate slow- and fast-flipping G-G base pairs. Biochemistry, 60, 1097-1107.


44.     Lixia Yang, Desiree-Faye Kaixin Toh, Manchugondanahalli S. Krishna, Zhensheng Zhong, Yiyao Liu, Shaomeng Wang, Yubin Gong and Gang Chen* (2020) Tertiary Base Triple Formation in the SRV-1 Frameshifting Pseudoknot Stabilizes Secondary Structure Components. Biochemistry, 59, 4429-4438.


43.     Maity, A., Winnerdy, F.R., Chang, W.D., Chen, G., and Phan, A.T.* (2020) Intra-locked G-quadruplex structures formed by irregular DNA G-rich motifs. Nucleic Acids Res. 48, 3315-3327. (IF: 11.561)


42.     Ong, A.A.L., Tan, J., Bhadra, M., Dezanet, C., Patil, K.M., Chong, M.S., Kierzek, R., Decout, J.L., Roca, X., and Chen, G.* (2019) RNA secondary structure-based design of antisense peptide nucleic acids for modulating disease-associated aberrant tau pre-mRNA alternative splicing. Molecules, 24, 3020. (IF: 3.098)


41.  Krishna, M.S., Wang, Z., Bowry, J., Wang, Z., Zheng, L., Bowry, J., Ong, A.A.L., Mu, Y., Prabakaran, M., and Chen, G.* (2019) Incorporating G-C Pair-Recognizing Guanidinium into PNAs for Sequence and Structure Specific Recognition of dsRNAs over dsDNAs and ssRNAs. Biochemistry, 58, 3777-3788. (IF: 2.997)


40.  Ong, A.A.L., Toh, D.F.K., Krishna, M.S., Patil, K.M., Okamura, K., and Chen, G.* (2019) Incorporating 2-thiouracil into short dsRNA-binding PNAs for enhanced recognition of A-U pairs and for targeting a microRNA hairpin precursor. Biochemistry, 58, 3444-3453. (IF: 2.997)


39.  Krishna, M.S., Toh, D.F.K., Meng, Z., Ong, A.A.L., Wang, Z., Lu, Y., Xia, K., Prabakaran, M., and Chen, G.* (2019) Sequence- and structure-specific probing of RNAs by short nucleobase-modified dsRNA-binding PNAs incorporating a fluorescent light-up uracil analog. Anal. Chem., 91, 5331-5338. (IF: 6.042)


38.  Tan, J., Yang, L., Ong, A.A.L., Shi, J., Zhong, Z., Lye, M.L., Liu, S., Lisowiec-Wachnicka, J., Kierzek, R., Roca, X.,* Chen, G.* (2019) A disease-causing intronic point mutation C19G alters tau exon 10 splicing via RNA secondary structure rearrangement. Biochemistry, 58, 1565-1578. (IF: 2.997)


37.  Kesy, J., Patil, K.M., Kumar, S.M. Shu, Z., Yee, Y.H., Zimmermann, L., Ong, A.A.L., Toh, D.F.K., Krishna, M.S., Yang, L., Decout, J.L., Luo, D., Prabakaran, M.,* Chen, G.,* and Kierzek, E.* (2019) A short chemically modified dsRNA-binding PNA (dbPNA) inhibits influenza viral replication by targeting viral RNA panhandle structure. Bioconjugate Chem., 30, 931-943. (IF: 4.485)


36.  Ong, A.A.L., Toh, D.F.K., Patil, K.M., Meng, Z., Yuan, Z., Krishna, M.S., Devi, G. Haruehanroengra, P., Lu, Y., Xia, K., Okamura, K., Sheng, J., and Chen, G.* (2019) General recognition of U-G, U-A, and C-G pairs by double-stranded RNA-binding PNAs incorporating an artificial nucleobase. Biochemistry, 58, 1319-1331. (IF: 2.997)


35.  Yang, L., Zhong, Z., Tong, C., Jia, H., Liu, Y., and Chen, G.* (2018) Single-molecule mechanical folding and unfolding of RNA hairpins: Effects of single A-U to A∙C pair substitutions and single proton binding and implications for mRNA structure-induced −1 ribosomal frameshifting. J. Am. Chem. Soc. 140, 8172-8184. (IF: 14.357)


34.  Patil, K.M., Toh, D.F.K., Yuan, Z., Meng, Z., Shu, Z., Zhang, H., Ong, A.A.L., Krishna, M.S., Lu, L., Lu, Y.,* and Chen, G.* (2018) Incorporating uracil and 5-halouracils into short peptide nucleic acids for enhanced recognition of A-U pairs in dsRNAs. Nucleic Acids Res. 46, 7506-7521. (IF: 11.561)


33.  Maraswami, M., Chen, G.,* and Loh, T.P.* (2018) Iridium(III)-catalyzed selective and mild C-H amidation of cyclic N-sulfonyl ketimines with organic azides. Adv. Synth. Catal. 360, 416-421. (IF: 5.123)


32.  Puah, R.Y. Jia, H., Maraswami, M., Toh, D.F.K., Ero, R., Yang, L., Patil, K.M., Ong, A.A.L., Krishna, M.S., Sun, R., Tong, C., Huang, M., Chen, X., Loh, T.P., Gao, Y.G., Liu, D.X.,* and Chen, G.* (2018) Selective binding to mRNA duplex regions by chemically modified peptide nucleic acids stimulates ribosomal frameshift. Biochemistry 57, 149-159. (IF: 2.997)

Invited for the Future of Biochemistry special issue, 
Cover highlight of 44 faces representing the Future of Biochemistry,  Editorial


31.  Maraswami, M., Pankajakshan, S., Chen, G.,* and Loh, T.P.* (2017) Palladium-catalyzed direct C-H trifluoroethylation of aromatic amides. Org. Lett. 19, 4223-4226. (IF: 6.492)


30.  Chen, H., Jia, H., Tham, H.P., Qu, Q., Xing, P., Zhao, J., Phua, S.Z.F., Chen, G.,* and Zhao, Y.* (2017) Theranostic prodrug vesicles for imaging guided co-delivery of camptothecin and siRNA in synergetic cancer therapy. ACS Appl. Mater. Interfaces 9, 23536-23543. (IF: 8.097)


29.  Toh, D.F.K., Patil, K.M., and Chen, G.* (2017) Sequence-specific and selective recognition of double-stranded RNAs over single-stranded RNAs by chemically modified peptide nucleic acids. J. Vis. Exp. 127, e56221. (IF: 1.325)


28.  Zhong, Z., Yang, L., Zhang, H., Shi, J., Vandana, J., Lam, D.T.U.H., Olsthoorn, R.C.L., Lu, L., and Chen, G.* (2016) Mechanical unfolding kinetics of the SRV-1 gag-pro mRNA pseudoknot: possible implications for −1 ribosomal frameshifting stimulation. Sci. Rep. 6, 39549. (IF: 4.122)


27.  Toh, D.F.K., Devi, G., Patil, K.M., Qu, Q., Maraswami, M., Xiao, Y., Loh, T.P., Zhao, Y.,* and Chen, G.* (2016) Incorporating a guanidine-modified cytosine base into triplex-forming PNAs for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex. Nucleic Acids Res. 44, 9071-9082. (IF: 11.561)


26.  Tan, J., Ho, J.X.J., Zhong, Z., Luo, S., Chen, G., and Roca, X.* (2016) Noncanonical registers and base pairs in human 5’ splice-site selection. Nucleic Acids Res. 44, 3980-3921. (IF: 11.561)


25.  Guo, S., Kierzek, E., Chen, G., Zhou, Y.J., and Wong, S.K.* (2015) TMV mutants with poly(A) tracts of different lengths demonstrate structural variations in 3′UTR affecting viral RNAs accumulation and symptom expression. Sci. Rep. 5, 18412. (IF: 4.122)


24.  Zhong, Z., and Chen, G.* (2015) How RNA catalyzes cyclization. Nat. Chem. Biol. 11, 830-831. (IF: 13.843)


23.  Zhong, Z., Soh, L.H., Lim, M.H., and Chen, G.* (2015) A U∙U pair-to-U∙C pair mutation-induced RNA native structure destabilization and stretching-force-induced RNA misfolding. ChemPlusChem 80, 1267-1278. (IF: 3.205)


22.  Zhang, L., Liu, H., Shao, Y.,* Lin, C., Jia, H., Chen, G.,* Yang, D.,* and Wang, Y. (2015) Selective lighting up of epiberberine alkaloid fluorescence by fluorophore-switching aptamer and stoichiometric targeting of human telomeric DNA G-quadruplex multimer. Anal. Chem. 87, 730-737. (IF: 6.042)


21.  Devi, G., Zhou, Y., Zhong, Z., Toh, D.F.K., and Chen, G.* (2015) RNA triplexes: From structural principles to biological and biotech applications. Wiley Interdiscip. Rev. RNA 6, 111-128. (IF: 5.844)


20.  Ma, X., Devi, G., Qu, Q., Toh, D.F.K., Chen, G.,* and Zhao, Y.* (2014) Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles. Bioconjugate Chem. 25, 1412-1420. (IF: 4.485)


19.  Devi, G., Yuan, Z., Lu, Y., Zhao, Y.,* and Chen, G.* (2014) Incorporation of thio-pseudoisocytosine into triplex-forming peptide nucleic acids for enhanced recognition of RNA duplexes. Nucleic Acids Res. 42, 4008-4018. (IF: 11.561)


18.  Zhou, Y., Kierzek, E., Loo, Z.P., Antonio, M., Yau, Y.H., Chuah, Y.W., Geifman-Shochat, S., Kierzek, R.,* and Chen, G.* (2013) Recognition of RNA duplexes by chemically modified triplex-forming oligonucleotides. Nucleic Acids Res. 41, 6664-6673. (IF: 11.561)


17.  Tinoco, I., Jr.,* Chen, G., and Qu, X. (2010) RNA reactions one molecule at a time, in RNA Worlds, (Gesteland, R.F., Cech, T.R., and Atkins, J.F., Eds.), Cold Spring Harbor Laboratory Press. Cold Spring Harb. Perspect. Biol. 2, a003624. doi: 10.1101/cshperspect.a003624. (IF: 9.274)


16.  Chen, G., Chang, K.Y., Chou, M.Y., Bustamante, C., and Tinoco, I., Jr.* (2009) Triplex structures in an RNA pseudoknot enhance mechanical stability and increase efficiency of –1 ribosomal frameshifting. Proc. Natl. Acad. Sci. USA 106, 12706-12711. (IF: 9.504)


15.  Chen, G., Kennedy, S.D., and Turner, D.H.* (2009) A CA+ pair adjacent to a sheared GA or AA pair stabilizes size-symmetric RNA internal loops. Biochemistry 48, 5738-5752. (IF: 2.997)


14.  Chen, G., Wen, J.D., and Tinoco, I., Jr.* (2007) Single-molecule mechanical unfolding and folding of a pseudoknot in human telomerase RNA. RNA 13, 2175-2188. (IF: 4.490)


13.  Chen, G., Kierzek, R., Yildirim, I., Krugh, T.R., Turner, D.H.,* and Kennedy, S.D. (2007) Stacking effects on local structure in RNA: Changes in the structure of tandem GA pairs when flanking GC pairs are replaced by isoG-isoC pairs. J. Phys. Chem. B 111, 6718-6727. (IF: 3.146)


12.  Shankar, N., Kennedy, S.D., Chen, G., Krugh, T.R., and Turner, D.H.* (2006) The NMR structure of an internal loop from 23S ribosomal RNA differs from its structure in crystals of 50S ribosomal subunits. Biochemistry 45, 11776-11789. (IF: 2.997)


11.  Chen, G., Kennedy, S.D., Qiao, J., Krugh, T.R., and Turner, D.H.* (2006) An alternating sheared AA pair and elements of stability for a single sheared purine-purine pair flanked by sheared GA pairs in RNA. Biochemistry 45, 6889-6903. (IF: 2.997)


10.  Chen, G., and Turner, D.H.* (2006) Consecutive GA pairs stabilize medium-size RNA internal loops. Biochemistry 45, 4025-4043. (IF: 2.997)


9.  Chen, G., Znosko, B.M., Kennedy, S.D., Krugh, T.R., and Turner, D.H.* (2005) Solution structure of an RNA internal loop with three consecutive GA pairs. Biochemistry 44, 2845-2856. (IF: 2.997)


8.  Chen, G., Znosko, B.M., Jiao, X., and Turner, D.H.* (2004) Factors affecting thermodynamic stabilities of RNA 3 × 3 internal loops. Biochemistry 43, 12865-12876. (IF: 2.997)


7.  Cui, Y.,* Chen, G., Ren, J., Shao, M., Xie, Y., and Qian, Y.* (2003) Solvothermal syntheses of β-Ag2Se crystals with novel morphologies. J. Solid State Chem. 172, 17-21. (IF: 2.179)


6.  Cui, Y.,* Chen, J., Chen, G., Ren, J., Yu, W., and Qian, Y. (2001) Bis(2,2-bipyridine-N,N')tetra-µ-chloro-tetracopper(I). Acta Crystallogr. Sect. C 57, 349-351. (IF: 8.678)


5.  Shao, M., Mo, M., Cui, Y., Chen, G., and Qian, Y.* (2001) The effect of agitation states on hydrothermal synthesis of Bi2S3 nanorods. J. Cryst. Growth 233, 799-802. (IF: 1.573)


4.  Cui, Y.,* Ren, J., Chen, G., Qian, Y.,* and Xie, Y. (2001) A simple route to synthesize MInS2 (M = Cu, Ag) nanorods from single-molecule precursors. Chem. Lett., (3) 236-237. (IF: 1.485)


3.  Cui, Y.,* Ren, J., Chen, G., Yu, W., and Qian Y. (2000) Poly[lead(II)-µ-4,4-bipyridine-N:N'-di-µ-bromo]. Acta Crystallogr. Sect. C 56, E552-E553. (IF: 8.678)


2.  Cui, Y., Chen, G., Ren, J., Qian, Y.,* and Huang, J.* (2000) Syntheses, structures and magnetic behaviors of di- and trinuclear pivalate complexes containing both cobalt(II) and lanthanide(III) ions. Inorg. Chem. 39, 4165-4168. (IF: 4.700)


1.  Cui, Y.,* Zhang, X., Zheng, F., Ren, J., Chen, G., Qian, Y., and Huang J. (2000) Two mixed-metal carboxylate-base adducts. Acta Crystallogr. Sect. C 56, 1198-1200. (IF: 8.678)


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